Trypanosomasis (surra) still a serious problem in camel husbandry world widly. It causes considerable economic losses in many camel-rearing regions. A little date for Real Time polymerase chain reaction (PCR) for rapid detection Trypanosoma evansi of blood from naturally infected camels were puplished. In the present study 242 blood samples from clinically suspected camels to surra from four province of Saudia (Al- Riyadh, Al- Qassim, Alharig, and Al-Dawadmi) were collected and used for further examination. Real Time PCR-based assay for blood samples were conducted in direct detection of T. evansi DNA from the collected blood samples using specific primer set (TR3/TR4) derived from nuclear repetitive gene of T.evansi and SYBER Green1 fluorescent dye have been used. The melting peak chart of the positive samples showed one single peak at an average Tm of 85.0^oC. The performed real time PCR technique was found accurate method for diagnosis of trypanosoma infection of camels where; 102 (42.15%) out of 242 were positive. It is concluded that camel trypanosomiasis in Sudia apparently caused by the present single parasite species T. evansi and detection of other typanosomes species are recommonded. In addition, the disease is highly prevalent in the country, which strengthens the need to change control policies and institute measures that help prevent the spread of the parasite. To our knowledge, this is the first molecular diagnosis report, which gives a picture of camel trypanosomiasis covering large geographical areas in Saudi Arabia.