Toxoplasma gondii is one of the important zoonotic parasites of worl-dwide zoological and geographical distribution. All animal species act as intermediate hosts to the Toxoplasma gondii, except feline species which act as a definitive host. A total of482 serum samples (182 male and 300 female) were collected during 2009/2010 from native camels (3-6 years), represented five different provinces of Saudi Arabia to monitoring serologically for Toxoplasma gondii antibodies by the indirect enzyme linked immuno-sorbant assay (ELISA). A total of 219 (45.44%) camel sera were serologically positive for toxoplasmosis. The prevalence was higher in female (47%) than male (42.86%).The prevalence was high in Al-Riyadhand Alharig (50.68% and 47.15%) comparing to other areas: Wady Al-Dawaser (41.84%), Dar-maa (40.58%) and Al-Solyel (38.64%) respectively. This study suggests widespread infection with Toxoplasma gondii among camels, a finding that warrants a closer look into the possible ways infection are acquired by camels in their arid environment, its economic impact, as well as its public health significance, especially among the nomads who consume cameline milk and raw liver.