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176863

EPIDEMIOLOGICAL ASPECT OF BOVINE VIRAL DIARRHEA VIRUS- II INFECTION IN DAIRY CATTLE

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Last updated: 05 Jan 2025

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Abstract

The purpose of this study was to characterize the clinical and virologic features of the mucosal and thrombocytopenic BVDV infection in infected dairy cattle. Strategy of examination included clinical examination of diseased animals, serum samples (n=31) represented all diseased cattle, milk samples (n=8) represented clinically diseased cattle and vaginal swabs (n=8) from the hemorrhagic diseased and aborted cattle were tested. An international reference strain (NADL: National Animal Disease Laboratory) and local cytopathic BVDV genotype-II strain (Behera-CP 58/99) were used as positive controls. The virus was isolated from the 8 vaginal swabs and 5 out of the 8 milk samples. All the isolates were ncp that no CPEs were noticed over the 3 passages. By specific BVDV genotype -II monoclonal antibodies (MAbs) against gp53, the viral antigen was identified using Fluorescence isothiocyanate (FITC)-conjugated anti-bovine IgG (specific intra cytoplasmic fluoresce granules) and horseradish peroxidase (HRP)-conjugated anti-bovine IgG (specific intra cytoplasmic brown granules) were detected. By enzyme linked immunosorbant assay (ELISA) technique, all serum samples were positive against the BVDV that had neutralizing antibodies titer ranges from ≥ 1/128 to ≥ 1/512. In conclusion, BVDV type -II do exist in cattle population and the understanding the molecular epidemiology is fundamental. Improved diagnostic and control strategies are essential to reduce losses inflected by BVDVs infection.

DOI

10.21608/avmj.2011.176863

Keywords

Key words: Bovine viral diarrhea virus- II, Epidemiological aspect, isolation, Immunohistochemistry

Authors

First Name

Y.G.M.

Last Name

ABD EL-HAFEIZ

MiddleName

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Affiliation

Virology Research Unit, Animal Reproduction Research Institute (ARRI), Giza, Egypt.

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Orcid

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First Name

A.M.

Last Name

EL-SHERIF

MiddleName

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Affiliation

Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University

Email

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City

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Orcid

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First Name

K.N.

Last Name

METIAS

MiddleName

-

Affiliation

Department of Animal Medicine, Faculty of Veterinary Medicine, Beni-Suef University

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Orcid

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Volume

57

Article Issue

130

Related Issue

24338

Issue Date

2011-07-01

Receive Date

2021-06-11

Publish Date

2011-07-01

Page Start

1

Page End

11

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 57_Issue 130_Pages 1-11.pdf

PDF . 464.1kB

Link

https://avmj.journals.ekb.eg/article_176863.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=176863

Order

15

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

EPIDEMIOLOGICAL ASPECT OF BOVINE VIRAL DIARRHEA VIRUS- II INFECTION IN DAIRY CATTLE

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Article

Created At

23 Jan 2023