The goal of the present study was to screen the the bacteriological, pathological and biochemical changes associated with the urinary tract affections in cattle and buffaloes and also to evaluate the diagnostic importance of urinary enzymes in urinary tract affections. A total number 75animals, (45) cattle, and (30) buffaloes, were subjected to this study. Fifteen cattle and 10 buffaloes were considered the control groups. These animals were randomly selected. Bacteriological examinations reveald that 80% of examined urine samples and 77.3% of examined organs samples were positive for bacterial isolation. Samples from (kidney, ureter and urinary bladder) used for histopathological examination. This study, discussed pathological changes and relation to changes in urine constutants, with an increase in the urine and serum enzymes. Bacterial isiolated strains were, E coli resembled 34.66%, Proteous sp 32 %, Streptococcus feacalis 20 %, Staphylococcus aureus, 18.6%, Klebsilla 18.6%, and Salmonela sp.2.6%. According to the histopathological findings, the animals under investigation were suffered from pyelonephritis, uretritis and cystitis. Urine analysis is helpful in diagnosis of pyelonephritis, uretritis and cystitis. There were a significant increase in serum levels of AST, ALT, LDH and ALP enzymes activities. Significant increase in AST, and LDH enzymes activities in urine of cattle and buffaloes. There were also, a marked increases in both ALT and ALP enzymes activities levels in urine of diseased animals in comparing with the normal levels. Increased enzyme activities are considered reliable indicators of pathological changes in various tissues due to the realease of intracellular enzymes from damaged tissue. Increased activities of serum enzymes lead to increased activities of these enzymes in urine.