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176858

PROTECTIVE EFFECT OF AQUAEOUS GREEN TEA EXTRACT ON TOXICITY CAUSED BY SODIUM NITRITE

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Last updated: 28 Dec 2024

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Abstract

Food additives are substances internationally added to food, this may be natural or synthetic. The safety of repeated exposure to permitted synthetic food additives (colorants or preservatives) has been questioned. Sodium nitrite (NaNO2, a food preservative agent) was used at 80 mg/kg/day and orally administered to the rats either alone or in conjugation with aqueous green tea extract (GTE, 10%) for 2 months. Blood and organs samples were collected for biochemical and histopathological examinations. Ingestion of NaNO2, induced a significant decline in blood glutathione reduced (GSH), plasma total antioxidant capacity (TAC) and glutathione -S- transferase (GST). Furthermore, significant increase was recorded in the level of serum lipid peroxide (MDA) and plasma nitric oxide (NO). Moreover, serum alanine transaminase (ALT), aspartate aminotransferase (AST) as well as bilirubin levels were increased significantly, while albumin level was decreased. Also, serum urea and creatinine levels were significantly increased. Plasma lactate dehydrogenase (LDH) and serum Creatine phosphokinase (CPK) activities were increased. Histopathological examination in sodium nitrite group rats revealed marked alterations in liver as apoptosis, periportal necrosis Sometimes, there were areas of focal necrosis which infiltrated with mononuclear cells giving the picture of granuloma like lesions, in addition to severe vacuolar degeneration. Also, kidney showed severe vacuolar degeneration and massive areas of tubular necrosis. Furthermore, heart showed necrosis of myocardial muscle cells. Fortunately, administration of green tea extract in conjugation with sodium nitrite showed significant amelioration of the investigated parameters and pathological changes. The results indicated that green tea extract has a potential to be developed as a preventive extract against sodium nitrite induced toxicity and the mechanism involved in the protection could be due to its antioxidant activity.

DOI

10.21608/avmj.2011.176858

Keywords

Key words: Sodium nitrite, Green tea extract, toxicity, Antioxidant activity

Authors

First Name

GHADA

Last Name

M GOMAA

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Affiliation

Dept. of Forensic Medicine and Toxicology, Fac. of Vet. Med., Kafrelsheikh University, Egypt

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First Name

EMAN

Last Name

A. ABD ELAZIZ

MiddleName

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Affiliation

Dept. of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

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Volume

57

Article Issue

130

Related Issue

24338

Issue Date

2011-07-01

Receive Date

2011-05-02

Publish Date

2011-07-01

Page Start

1

Page End

25

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_176858.html

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https://avmj.journals.ekb.eg/service?article_code=176858

Order

11

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

PROTECTIVE EFFECT OF AQUAEOUS GREEN TEA EXTRACT ON TOXICITY CAUSED BY SODIUM NITRITE

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Article

Created At

23 Jan 2023