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176945

COMPARATIVE STUDIES BETWEEN INDIRECT HEMAGGLUTINATION (IHA) TEST AND ENZYME LINKED IMMUNOSORBENT ASSAYS (ELISA) TEST FOR DETECTION OF SERUM ANTIBODY LEVELS AGAINST INFECTIOUS BURS

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Last updated: 05 Jan 2025

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Abstract

This study was done to compare between IHA test and ELISA test as a serological test for detection of serum antibody levels against Infectious Bursal Disease Virus (IBDV) in Commercial Broilers, 300 Serum samples were collected from different commercial broiler farms and slaughter Shops from in and around Beni-suef Governorate. These samples were divided into three groups according to the age of the birds i.e. 0-3 weeks (100 samples), 3-6 weeks (100samples) and 6-8 weeks (100 samples).ELISA and Indirect Hemagglutination (IHA) test were used to titrate the serum antibodies against infectious bursal disease virus. Hyper immune serum was raised in laboratory rabbits against IBDV vaccine (D-78). It was used to identify and confirm the IBD virus from bursal tissues isolated from the IBD affected flocks using the qualitative tests i.e. agar gel precipitation suspension was prepared and used to determine the antibody titer. Results indicated that out of 300 test serum samples 195 (65%) were positive. The maximum positive samples belonged to 6-8 weeks of age (89%) followed by 3-6 weeks (73%) and 0-3 weeks (33%), respectively while the result of ELISA test of high and low titers were 18% and 82 % at 0-3 week of age then at age 3-6 weeks the high titers were 35% and low titers were 65% and finally high ELISA titers 69% and low titers 31%at age 6-8 weeks.

DOI

10.21608/avmj.2011.176945

Authors

First Name

AL-H.M.

Last Name

DAHSHAN

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Affiliation

Dept. of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University

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First Name

A.S.

Last Name

HUSSIEN

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Affiliation

Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University

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Volume

57

Article Issue

131

Related Issue

24337

Issue Date

2011-10-01

Receive Date

2021-07-19

Publish Date

2011-10-01

Page Start

1

Page End

12

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_176945.html

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https://avmj.journals.ekb.eg/service?article_code=176945

Order

27

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

COMPARATIVE STUDIES BETWEEN INDIRECT HEMAGGLUTINATION (IHA) TEST AND ENZYME LINKED IMMUNOSORBENT ASSAYS (ELISA) TEST FOR DETECTION OF SERUM ANTIBODY LEVELS AGAINST INFECTIOUS BURSAL DISEASE (IBD) VIRUS IN COMMERCIAL BROILERS

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Article

Created At

23 Jan 2023