The study was conducted to evaluate the ability of Aqueous Garlic Extract (AGE) for inhibition the growth of Salmonella typhimurium as a probable cause of arthritis in rabbits in vitro and in vivo study. Results revealed that AGE (contain 201.2µg/ml allicin) have excellent antibacterial activity against this strain at ≥60% concentration, while the concentration ≥20% showed moderate activity, M oreover concentration of <20% show no effect in vitro. The in vivo study was explained the infectious dose of Salmonella typhimurium that induce arthritis in experimental animals was ≥ 1×10⁵ C.F.U/ml when injected intrarticularly. Garlic extract was administrated to treat experimental induced arthritis for 5 days by using different doses subcutaneously. It have been shown that a dose rate of 100mg/kg B.W S.C were more effective and have no adverse effects. Some bacteriology, serology and hematological tests were used as indicators to confirm infection and evaluation of treatment in rabbits.