Alloxan is known to induce diabetes mellitus in the experimental animals. The objective of this study is to evaluate the biochemical alterations resulted from alloxan- induced diabetes in the local breed of donkey males. Two groups of donkey males (n=7 each group) were subjected to the experiment, the 1^st one considered as a non diabetic control whereas the 2^nd injected with alloxan monohydrate (100 mg.     kg^-1) with a single dose. Results of serum biochemical analysis along with 15 days from alloxan injection revealed in addition to the hyperglycemia, a significant (p≤0.05) elevation in total cholesterol, triglycerides, total protein, albumin, creatinine, blood urea nitrogen, inorganic phosphate, malondialdehyde serum concentration as well as increased activity of serum enzymes alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine phosphokinase and γ- glutamyl transferase in respect to both control or baseline values. Serum calcium and glutathione concentration was declined as a result of experimental diabetes in male donkey with no significant changes in total bilirubin, hemoglobin, sodium and potassium concentrations. In conclusion, administration of a single dose of alloxan to the male donkey produced a reproducible model of diabetes mellitus which negatively affects the serum lipids, proteins, liver enzymes, kidney function and the markers of oxidative stress.