Ascitis is world wide syndrome in growing broiler chickens responsible for significant economic losses. This study was conducted to investigate the bacterial, mycotoxin causes or in association with ascites and the biochemical changes. 60 farms of broiler chickens aged 1–6 week with complain of ascites in some members. Random sample of ascetic birds from each were examined and sample of apparently healthy as control. Ration samples were taken when mycotoxicosis were suspected. The study claimed that 86.7 % of cases were bacteriologically positive. E. coli was the predominant isolate from ascetic cases 53 % of the positive cases, followed by Staph. aureus 26 %. Members of Enterobactereacea Proteus, Klebsiella and Citrobacter Spp. were isolated in 23.1, 19.2, 13.5 %, respectively, as a sole isolate from ascetic cases. E. coli, Staph. aureus, Proteus, Klebsiella and Citrobacter were in incidence of 30.8,  11.5, 11.5, 7.5 and 3.8 %, respectively, with total incidence 65.3 %. Mixed infection represents 34.7 %. The gained E. coli were tested for pathogenicity and invasiveness as denoted 57.1 % of cases were positive for Congo red. The examined ration samples of some ascetic broiler flocks revealed aflatoxin and ochrotoxins with mean value of 12.38 and 6.36, respectively. The biochemical results revealed significant variations in electrolytes, minerals, liver enzymes and kidneys function between the bacterial & mycotoxin, bacterial, ascetic with no infection in comparison with apparently healthy broiler chicks.