Meats often contains micro-organism which may cause disease, Including Klebsiella spp. Which cause wide range of disease states, notably pneumonia, urinary tract infections (UTIs), and bacteremia. The aim of this study was collected 100 samples of meats (Calf, sheep, chicken) from the meats shops present in the different area in Damascus and countryside in a sterile container, for isolated Klebsiella and growing it on Selective Media  to identify and characterize its morphology, biochemical and Molecular Characteristics by using Polymerase chain reaction PCR. Colonies of the microorganisms were counted on difference media then isolated from a selective media. The colonies of Klebsiella isolated are circular, dome-shaped. 3-4 mm diameter with mucoid aspect, stickiness and surrounded by capsule.  Our results showed that 46% of examined specimen contained Klebsiella spp.The results showed that it can investigate of Klebsiella in meats samples by biochemical reactions and polymerase chain reaction. In next study we will be produced primers To differentiate between two types of Klebsiella           (K. pneumoniae and K. oxytoca).