171611

STUDIES ON SOME FOOD POISONING BACTERIA IN RAW MILK SOLD IN SHARKIA GOVERNORATE

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Last updated: 05 Jan 2025

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Abstract

One hundred raw farm bulk milk samples were collected from Sharkia Governorate. A survey was conducted to determine the incidence of food poisoning bacteria in raw milk and experiments were carried to determine antibiotic sensitivity of Campylobacter jejuni & Yersinia enterocolitica and proteolytic & lipolytic activities of isolated Pseudomonas spp. Out of the 100 raw farm bulk milk samples tested 1 (1%), 1 (1%) and 2 (2.0%) were found to contain, Campylobacter jejuni against Karmali, Columbia and modified Preston agar, respectively. Serotyping of Campylobacter jejuni isolated from the examined raw milk samples resulted in two strains were belonged to Campylobacter jejuni serotype 2 while only one was Campylobacter jejuni serotype 1. Antibiotic sensitivity revealed that all the tested Campylobacter jejuni isolates were 100% sensitive to Genitamycin and Chloramphenicol while none were sensitive to Cefoperazone and Ampicillin. Yersinia enterocolitica and Yersinia krestensenii could be detected in 7% and 4% respectively when cultured on bile oxalate sorbose enrichment broth, while using Yersinia enrichment broth (YEB) 7.33% and 4% of milk samples were positive for Yersinia enterocolitica and Yersinia krestensenii respectively. 50% of the examined samples were contaminated with Pseudomonas spp. The high level of contamination was 0.5×  108 ; the low level was 2.3  ×102 and the mean value was 1.8 × 104 ± 0.5 × 104. Also, it is found that out of the examined 100 raw bulk milk samples 2 (2%) were positive for E. coli.

DOI

10.21608/avmj.2013.171611

Keywords

Key words: Food Poisoning, Raw milk, antibiotics

Authors

First Name

ABD-EL-KALIEK,

Last Name

A.A.

MiddleName

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Affiliation

Animal Health Research Institute Zagazig Provincial Laboratory

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City

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First Name

MASOUD,

Last Name

S.E.

MiddleName

-

Affiliation

Animal Health Research Institute Zagazig Provincial Laboratory

Email

-

City

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Orcid

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First Name

MONA,

Last Name

T.A. RASLAN

MiddleName

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Affiliation

Animal Health Research Institute Zagazig Provincial Laboratory

Email

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City

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Volume

59

Article Issue

138

Related Issue

24330

Issue Date

2013-07-01

Receive Date

2013-03-31

Publish Date

2013-06-12

Page Start

49

Page End

54

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_171611.html

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https://avmj.journals.ekb.eg/service?article_code=171611

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7

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

STUDIES ON SOME FOOD POISONING BACTERIA IN RAW MILK SOLD IN SHARKIA GOVERNORATE

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Article

Created At

23 Jan 2023