Q fever is a rickettsial disease caused by Coxiella burnetii bacterium, This study was performed to investigate the existence of Q fever in human in the middle region of the Syrian Arab Republic, the study concentrated on two regions which are Hamah city and Mysiaf, in addition to some places between them, using PCR test. Coxiella burnetii had been detected as a causative agent of Q fever with consideration that the samples were taken randomly. The prevalence rate reached at (68%) of the human groups included in the study. This result requires High degrees of caution in order to control this disease.