Previously Pasteurella multocida was isolated and genotyped by the author from different outbreaks in rabbits at Sharkia Governorate, Egypt. In this work, development of avaccine was carried out in the laboratory by sonication of the isolated bacteria genotype A, B and D. Two experiments were conducted for evaluation of the trialed vaccine. The first one was carried out for determination of the protective dose of the vaccine and the second experiment for testing of its immune competency with commercial vaccine. Immunogenic response was evaluated serologically by ELISA and protection percentage on challenge against infection. Results revealed that sonicated vaccine containing 4x10⁹ colony forming unit (cfu)/ml provoked high level of immunity. On comparison with commercial vaccine, geometric mean antibody titre of the prepared vaccine was statistically higheralong 4 weeks post vaccination. Moreoverit produced 100% protection against challenge while commercial vaccine revealed 80% protection. Unvaccinated control grouphad zero protection. It is concluded that immunization of rabbits with vaccine prepared by sonication of genotyped field P multocida isolate would besupportive means for avoidance of pasteurollosis in rabbitaries.