The purpose of this study aimed to investigate the influence of different doses of probiotic (Saccharomyces cerevisiae) co-administrated with Aflatoxin B₁ in male rabbits and study the possible protection against its adverse effect. A total number of 20 adult New Zealand (NZ) male rabbits were randomly divided into 4 groups: (G І) untreated control group, (G II) treated with AFs (30ug/kg b.wt.), (G III) treated with Sc (0.5% of ration /rabbit) + AFs (30ug/kg b.wt.), (G IV) treated with the Sc (1% of the ration/rabbit) + Afs (30ug/kg b.wt.) for 8 weeks.  There was significant depression of growth rate in the treated groups that were more pronounced in (G II) and (G IV) while there was some protection in (G III). Liver function which represented by the levels of ALT, AST and ALP enzymes was significantly affected by aflatoxin that were pronounced in groups received aflatoxin alone or in combination with the second dose of Sc, while Sc reduced this adverse effect in the 1^st dose of Sc group gradually by time. Total protein and albumin showed no significant effect on them except in the total protein level in group III (1st dose of Sc), while there were significant stimulatory effect on the level of globulin in all the periods in the same group. Aflatoxin B₁ caused significant bad effect on the kidney function represented by urea and creatinin levels especially in (GII and GIV), while there was some protection in (G III). There was significant stimulation of nitric oxide level in the (GII and GIV) as toxic effect than the other groups. There was significant stimulation of lysozyme activity in (G III) and (G IV) than other groups. There was significant damage of DNA in (G II) in 2nd week, while in the 8th week this damage extend in (G II) and (G IV) with great protection for the (G III). Significant stimulation in lymphocyte transformation occured in (G II) and (G III) than the other groups. Histopathological changes were observed in the testis, epididymis, liver and kidney tissues and the impact clearly reflected dependence on the type and the dose of treatment. The histopathological examination of testis varied from mild degeneration to a complete atrophy of seminiferous tubules and loss of spermatogenesis. Also, it induced hepatic and renal damages. Analysis of apoptosis showed massive necrosis and increased rate of apoptosis in group II and IV. In conclusion, Saccharomyces in low concentration was found to be safe and successful agent counteracting the Aflatoxin B1 toxicity and protect against the toxicity induced by it.