A total of 128 isolates of lactic acid bacteria (LAB) were isolated from hundred sausages samples collected from different supermarkets in Assiut City. The obtained isolates were identified bacteriologically as Pediococcus cervisiae (35), Enterococcus faecium (25), Lactobacillus brevis (12), Lactobacillus acidophilus (12), Lactobacillus bulgaricus (12), Lactobacillus fermetni (12), Streptococcus thermophilus (10) Lactococcus garvieae (10). Then they were screened for antagonistic activity against listeria monocytogenes, Staphylococcus aureus, Escherichia coli and Salmonella Typhimurium using agar well diffusions method. All these isolates exhibited antibacterial activity against L.monocytogenes and E.coli, the most active strain against both pathogens was Pediococcus cervisiae, whereas Enterococcus faecium, Streptococcus thermophilus and Lactococcus garvieae. were not active against, Staphylococcus. aureus, also Lactobacillus brevis, Lactobacillus acidophilus, Lactobacillus fermenti and Lactococcus garvieae were not active against Salmonella Typhimurium.