Bovine herpes virus type 1 (BHV-1) still the causes of great economic lossesin the livestock industry and trade because there are no available drug that proved to be fully effective against it. In this study, the antioxidant of selenium and/ or the vitamin E were evaluated as a natural anti-viral drugs. The antiviral activities against Bovine Herpesvirus type 1 (BHV-1) were evaluated by the reduction of the viral cytopathic effect 1nMadin-Darby Bovine Kidney cell line (MDBK) with, GSH (glutathione reduced) and MAD (malondialdehyde) assays. Selenium in concentrations 0.01 and 0.1 µM and / or Vit. E in concentrations 50 and 100 µM with two concentrations were added at different stages of the viral infection (pre and post treatment). This study concluded that the higher concentration of Selenium and Vit. E (0.1 µM+100µM respectively) reduced the Cytopathic Effect (CPE) of BHV-1 1n 50% and increased GSH level as well as decreased MDA level, in pre infection treatment assay.