A total of 150 of raw milk and yoghurt samples (75 of each) were obtained from different small daries and supermarkets in Assiut city, Egypt and were examind for presence of Staph aureus. The isolated strains were examined for the production of Staphylococcal enterotoxins (SEs) using the enzyme - linked immunosorbant assay (ELISA) method. 39(52%) out of 75 examind raw milk samples were positive for Staph aureus while the organism failed to detect in yoghurt. Out of 39 Staph aureus isolates 12 (30.8%) were positive for at least 1SE, out of which 5(41.7%) were positive for SEA 3(25%) for SEC, 2(16.7%) for SED. Two isolates of Staph aureus are able to produce more than one type of enterotoxin as one strain produce SEA&C and another produce C&D, both in percentage of 8.3%. It could concluded that examind milk samples were contaminated with enterotoxigenic strains of Staph. aureus and the public health hazards of SEs were also discussed.