Shiga-toxin-producing Escherichia coli (STEC) are the most important recently emerged group of food-borne pathogens. Among fifty EHEC strains, thirty strains were found as non O157 EHEC (60%) by serotyping. Also the highest incidence of O26 and O111 were 16% and 14%, respectively, meanwhile the lowest was of O128 (2%). Antibiotic resistance profiles for these strains showed 100% resistance to: sulfamethoxazole, Trimethoprime, chlormphencal, colistin, gentamycin and tetracycline. However, they showed 85% resistance to streptomycin and doxycycline, 80% resistance to cefotraxone while their resistance for amoxicillin clavulanic acid was 75%. PCR examination for twenty strains of EHEC non O157 revealed stx1 gene in 45%, stx2 in 65% while hly in 80% of the examined strains. Antimicrobial resistance genes for these strains confirmed that sulI, aadA and blaTEM resistance genes were detected in percentages of 85%, 75% and 60%, respectively. Recommendation for minimizing an excessive use of antibiotics in the veterinary field and periodically use of different antibiotics could overcome the problem of increased bacterial resistance.