Equine Herpes Virus-1(EHV-1) and 4 (EHV-4), both viruses are found in horse populations all over the world and leading to a feverish respiratory disease per annum among horses in areas with intense horse populations. Abortion in mares infection with EHV-4 was rarely, from another side mare may abort several weeks to months after clinical or subclinical infection with EHV-1. Nucleotide sequences of EHV-4 genes encoding each glycoprotein showed that the amino acid sequences (gB, gE, gG, gH, gK, gL, gM and gN of TH20p) resemble that of the reference genome (NS80567) except (gC, gD, gI and gP2) at certain positions. By constructing phylogenetic tree using glycoproteins sequences of EHV-4, EHV-4 reference genome and EHV-1 (strain 89c25p) revealed that there are two main clusters within each clusters.