Salmonellosis is one of the major bacterial problems in the poultry industry in Egypt and worldwide. Resistance to antimicrobial agents within nontyphoidal Salmonellae is a serious problem. The present study aimed to analyze some β-lactamase resistance genes in Salmonella isolates from broiler chicken. Five hundred samples were collected from diseased broiler chickens of different ages (3-6 weeks) from different farms in Assiut Governorate during the period from January 2015 to December 2015. Bacteriological examination showed that 26 salmonella isolates were recovered with a prevalence rate of 5.2% Serotyping of Salmonella isolates showed that S. Enteritidis S. Typhimurium, and S. Kentuky were identified at rates of 50%, 30.8% and 19.2%, respectively. Results of antibiogram showed that 18 salmonellaisolates (92.3%) were multidrug resistant. All isolates were screened for the presence of 2 β-lactamase resistance genes (bla_CTX and bla_CMY) using multiplex PCR. The overall prevalence was 14/26 (53.9%) for bla_CTX and 9/26 (34.6%) for bla_CMY.