Castration with ablation of scrotum was performed on six juvenile cats. Participated animals were generally anesthetized by and secured in dorsal recumbency. Ventral abdominal and perineal regions were scrubbed and disinfected. Two artery forceps were placed at the base of scrotum to secure testicles in place and to control hemorrhage. Scrotum was excised and removed. Spermatic cords of both testicles were ligated and transected. Testicles were removed and skin at the base of scrotum was sutured. The procedures were feasible, applicable, less time and effort consuming and cosmetically acceptable. Castration with ablation of scrotum in juvenile cats is a probable alternate castration approach in tomcats.