An increase in the consumption of milk shakes has been observed in recent years all over the world. In this work, the hygienic quality of chocolate and fruity milk shake was examined. This study was carried out to evaluate presence of E.coli, Enterococcus spp. and Staph. aureus in 50 samples of milk shake which were collected from different restaurants in Assiut city. The obtained results revealed that E.coli, Enterococcus spp < /em>. and S. aureus were isolated and confirmed by chemical tests in percentages of 16%, 24%; 20%, 44% and 12% and 24% of examined samples of chocolate and fruity milk shake, respectively. Concerning the mean values of total psychrotrophic count of chocolate milk shake and fruity milk shake were 9.9x103 and 1.2x104 CFU/g, respectively. Whereas, it was indicated that total yeasts and molds counts of the examined samples of chocolate milk shake and fruity milk shake were 3.2x102 and 2.1x103, respectively. The obtained results revealed that the average counts of Enterococci were 4.9x103 and 1.1x104 CFU/g in the examined chocolate and fruity milk shake samples, respectively. In general the analysis indicates unsatisfactory quality of milk shakes and need to be microbiologically controlled by regulatory authorities and the public health significance of isolated strains as well as suggested control measures were discussed.