This study investigated presence of Staphylococcus aureus and Salmonella typhimurium and some of their enterotoxins and virulence genes in live chicken and chicken meat in New Valley, Egypt. 200 broilers samples were collected as following;Liver and blood of 100 clinically diseased, 6 weeks old chicken were obtained from five broilers farms in New Valley governorate for surveillance of the microorganisms in poultry while, 100 freshly slaughtered broilers chicken which apparent healthy were collected from public live bird markets. The samples were meat specimens from the breast muscle, thigh muscle, liver, and blood (100 of each). The identified strains were screened for enterotoxin genes of Staph. aureus (SEA to SAE) genes. Prevalence of S. aureus in live chicken was higher than S. typhimurium whichwas 76% and 18%, respectively which was higher in blood than liver samples. On the other hand, the incidence of S. aureus was higher than S. typhimurium in apparently healthy and symptomatically diseased broilers. In chicken meat was with an overall incidence of 56.67%. The total prevalence of S. aureus and S. typhimurium was 51.33% and 5.33% respectively the highest microbial load was in liver samples followed by breast muscle then thigh muscle samples. S. aureus SAB gene was the only detected enterotoxins gene among chicken samples. Raw poultry meat available for consumers in Egypt often contaminated with pathogenic zoonotic bacterial agents.