The objective of this study was to calculate the liver activity index (LAI) and the liver functionality index (LFI) in buffaloes during post- partum period., 29 pregnant buffaloes were selected at 2^nd and 3^rd milk season from Ard El -Kheir farm in Assuit Governorate. Blood plasma was collected for determination of vit. A and serum were collected for determination of serum albumin, total cholesterol and total bilirubin. Samples were collected as the following: (-21d: -15d), (-14d: -8d), (-7d: -1d), calving day, 3 d P.P, 7 d P.P, 14 d P.P and 28 d P.P. After calculation of indexes, animals were classified into high and low groups (whenever the result is positive, it is considered high and whenever the result is negative, it is considered low). The study revealed highly significant difference in the mean values between high and low groups. The mean values ofalbumin revealed a non significant decrease in serum albumin in the low group compared with the high one at the same period in both indexes, except in LAI at calving day; there was a significant decrease in (LLAI) group compared with the (HLAI) one. Non significant decrease in the mean values of serum total cholesterol in the low group compared with the high one at the same period in both indexes, except in LAI at day 28 P.P.; there was a significant decrease in the (LLAI) group compared with the (HLAI) one. Non significant decrease in the mean values of vit. A in the (LLAI) group compared with the (HLAI) one at the same period except at day 14 P.P, there was a significant decrease in the (LLAI) group compared with the (HLAI) one. The mean values of total bilirubin revealedhighly significant difference between the (HLFI) and the (LLFI) groups during period of the study. A significant increase in total bilirubin at day 3 P.P in the (LLFI) group compared with the (HLFI) one and a significant increase (p < 0.05) in the (HLFI) at days 14 and day 28 P.P compared with the (LLFI).