Cows are occupied huge economic importance worldwide. Impaired fertility in cows due to absence of corpus luteum (CL) faces huge challenge. The aim of this study was to determine the effect of CL on ovarian biometry, follicular population, hormonal and metabolic content of serum and follicular fluid (FF) of cows. Blood samples and 48ovarieswerecollected from cows slaughtered at winter season and classified according to the presence or absence of CL into two groups, ovaries with and without CL. The diameter of antral follicles was taken and classified into three subgroups, small, medium and large follicles. FF aspirated from each follicle group. 17β-Estradiol (E₂), progesteron (P₄), glucose, total proteins, total cholesterol (TC) and nitric oxide (NO) were estimated. Result showed that the ovarian biometry was higher in right than left ovaries without significant difference. However, the total follicular populations and ovarian dimensions and their weights were significantly higher in ovaries with CL. The average number of small and medium follicles was also significantly increased in the ovaries with CL. However, the number of LF was higher in cows without CL compared to cows with CL. Glucose, Total cholesterol (TC), proteins, E₂ and P₄ concentrations were higher in serum than FF. Serum E₂ concentration was higher in LF and significantly reduced in ovaries had CL than without CL. On the other hand, P₄ concentration in FF was lower in LF and significantly increased in ovaries with CL. Ovaries had CL showed elevation of FF glucose level; however, TC, protein and NO concentrations were lower than ovaries without CL. Hence, we concluded that CL presence effects on both morphometric and metabolic conditions of cows^, ovaries.