Prevalence of mastitis in goats located in Nouqra valley, Aswan Governorate, by indirect method (California Mastitis Test) in paralleling with culturing and molecular (PCR) procedures for detection of Staphylococcus aureus as a major mastitis pathogen. A total of 148 raw milk samples were subsequently collected from apparently healthy native breed goats, with different age and parity, and subjected to California Mastitis Test. By CMT, our results indicated that 117 (79.05 %) of the tested—samples were positive. Culturally using Baired Parker media, pure strains of Staphylococcus aureus was isolated from CMT—positive samples with a percentage of 1.35% and 77.7% of the samples showed fair growth, which classified as other non-aureus staphylococci. Coagulase test revealed 7 (4.7%) samples were positive and 141 (95.3%) were negative. These isolates were molecularly tested using 16s Rrna (Staphylococcus genus specific), nuc gene (S.aureus species specific) and mecA gene (methicillin resistance gene) by Multiplex PCR Technique. Their results indicated that 87.5% were positive for 16s Rrna, 25% were positive for nuc gene, 75% were positive for mec A gene and 12.5% were negative. The in-vitro antibiotic sensitivity testing revealed that the resistant percentages to penicillin were surprise (100% resistance). Amoxicillin, cefaclore, colistin, oxolinic acid, neomycin, erythromycin, and lincomycin were also examined with various resistant results. Approx. 85 % (85.71%) of the tested strains were Ciprofloxacin—sensitive