Eighteen ejaculates were collected from three mature Arabian stallions (about 5 years of age) during breeding season (summer). Semen was collected, evaluated and extended with Tris-yolk-glucose, Milk-glucose and Non-fat Dried milk solids glucose extenders. The final extension rate was 1ml semen: 4ml extender. The extended semen was divided into 4 cervical tubes added with Glutathion (GSH) 0.0, 0.2, 0.4 and 0.8 mM/100ml ,. The extended semen was kept at 5 ºC for up to 48 hours. The percentages of sperm motility, dead spermatozoa, abnormal spermatozoa, and acrosome damage of spermatozoa were recorded. The results showed that, the percentage of motile stallion spermatozoa extended with tris-yolk-glucose or Non-fat Dried milk solids glucose extenders significantly (P<0.05) higher than Milk-glucose extender, while the percentages of dead spermatozoa, abnormal spermatozoa and acrosome damage of spermatozoa (%) significantly (P<0.05) lower than Milk-glucose extender. Supplementation of GSH at level of 0.4 mM to the extender increased significantly (P<0.05) the percentage of motile stallion spermatozoa, while decreased the percentages of dead spermatozoa, abnormal spermatozoa and acrosome damage of spermatozoa (%) as compared with the control samples in the different extenders, during storage at 5ºC. The prolongation of storage time at 5ºC decreased significantly (P<0.05) the percentages of sperm motility and increased significantly (P<0.05) the percentages of dead spermatozoa, abnormal spermatozoa and acrosome damage of spermatozoa (%) in the different extenders or GSH concentrations and free-GSH medium (control). In conclusion, it can be recommended to use of Tris-yolk-glucose extender or Non-fat Dried milk solids-glucose extender added with 0.4 mM glutathione /100 ml for enhancement of the Arabian stallion spermatozoa, during storage at 5 ºC.