Ducks are one of the popular household raised poultry in Egypt. Many Egyptian families in Upper and Lower Egypt are adopting the free range raising system where ducks are confined at night and let free at day time for swimming and foraging in the near canals and ponds. Thus, the present study aimed to survey a sample of free ranging ducks (100 birds) and Tilapia zillii fish (100 fish) for bacterial infection (E.coli, Salmonella spp < /em>., and Pseudomonas spp < /em>.), and for parasitic infection (Clinostomum species, Echinostoma species, ligula intestinalis species and Contraceacum species). E.coli was isolated with prevalence rates (47%) and (59%) fromducks, and Tilapia zillii, respectively. Pseudomonas spp. was isolated with prevalence rates of (15%, and 34%)from ducks, and Tilapia zillii, respectively. Salmonella spp was isolatedwith prevalence rates of (25%, and 9%) from ducks, and Tilapia zillii, respectively. Studying the prevalence of helminthes infestations revealed the isolation of Echinostoma species with prevalence rates of (13%, and 17%) from ducks, and Tilapia zillii, respectively. Clinostomum species was isolated with prevalence rates of (15%, and 39%) from ducks, and Tilapia zillii, respectively. Parasitic examination also revealed the isolation of Ligula intestinalis cestode with prevalence rates of (19%, and 22%) from ducks, and Tilapia zillii, respectively. Moreover, Contraceacum species was isolated with prevalence rates of (30%, and 46%) from ducks, and Tilapia zillii, respectively. Antimicrobial susceptibility patterns of bacterial isolates were studied by disc diffusion and the results revealed the high prevalence of multidrug resistance phenotypes among isolates. Studying genotypic attributes of isolates by PCR revealed the high prevalence of virulence, antimicrobial resistance and biofilm formation genes. The alarming findings highlighted the importance of conducting more detailed monitoring and investigation in the duck / fish interface and to raise public awareness for biosecurity practices to mitigate the imposed public health risk.