Liver fluke disease (fascioliosis) is an important parasitic disease may accountable for morbidity and mortality in most species of animals, like cattle, sheep and goat as well as other domestic ruminants. It occurs worldwide due to infection with liver fluke species. The study aimed to notice the gross, microscopic lesions and measured serum biochemical changes accompanied with acute bovine liver Fascioliosis of cattle in Kirkuk. From March to April 2018, fifteen infected bovine livers of different ages were collected from Kirkuk abattoir, and examined grossly. A noticeable rise in the level of serum AST, ALP and also ALT were observed. In gross examination several short vermiform cords were observed on the hepatic surface and the surface had a rough appearance. Some immature, wandering flukes were noted on the cut surface. Histopathologically, a wide range of liver lesions were found. The most important lesions were moderate to severe perihepatitis and haemorrhagic tracts on the hepatic surface. These lesions related to migratory channels filled with blood, fibrin and cellular debris. The present study results indicated that serum biochemical alterations were consistent with pathological lesions; hence the analysis of serum biochemical could be used in diagnosis of acute bovine fasciolosis with other tests.