A total of 200 samples of meat, minced meat, poultry meat and children diarrhea were collected from different shops in Aswan during 2018. These samples consist of 50 samples of each meat, minced meat, poultry meat and children diarrhea. Bacteriological examination of the samples was carried out for detection of Shiga toxins-producing Escherichia coli (STEC) followed by serological and genetic characterization for serotyping and for detection of some virulence genes including stx1, stx2 and eaeA genes. The incidence of E. coli in meat, minced meat, poultry meat and children diarrhea was (11/50) 22%, (9/50) 18%, (6/50) 12% and (6/50) 12%, respectively. Incidence of Shigatoxin – forming genes; stx1 ranged from 33-77% and stx2 ranged from 36-83%, while eaeA ranged from 16-36%. STEC were found in meat, minced meat, and poultry meat and transferred to children through contaminated food. Good processing procedures and sufficient temperatures should be confirmed during meat preparation to avoid infection and possible food poisoning from this dangerous pathogen.