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204806

MICROSCOPIC AND MOLECULAR STUDY OF BABESIA SPP. BABESIA BOVIS AND BABESIA BIGEMINA IN MOSUL CITY-IRAQ

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Last updated: 05 Jan 2025

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Abstract

The current study included examination of fifty blood samples which collected from both sexes and different ages of cattle in different areas of Mosul city, during the period from October 2017 to the end of November 2017 by using microscopical and polymerase chain reaction (PCR) techniques. The percentage of diagnosis of Babesia spp < /em>. Babesia bovis and Babesia bigemina by (PCR) 60% , 44%, 38% respectively , these percentage were higher than which recorded by microscopic examination 44%, 32%, 32% respectively. The results showed product of amplification on agarose gel 2%, 644, 350, 278 bp respectively, also this study showed that the alternative methods (not commercial) which used for extraction of DNA form the blood of cattle were evaluated as most efficient and obtained good concentration and purity of DNA which used in polymerase chain reaction and these methods characterized as most rapid, simple and dependable methods.

DOI

10.21608/avmj.2020.204806

Keywords

Babesia spp, Babesia bovis, Babesia bigemina, cattle, PCR

Authors

First Name

E.G.

Last Name

SULEIMAN

MiddleName

-

Affiliation

Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul-Iraq

Email

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City

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Orcid

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First Name

A.F.

Last Name

ALTAEE

MiddleName

-

Affiliation

Department of microbiology, college of veterinary medicine, University of Mosul, Mosul-Iraq

Email

ahlaam.altaee@gmail.com

City

-

Orcid

0000-0001-5862-3719

Volume

66

Article Issue

166

Related Issue

24251

Issue Date

2020-07-01

Receive Date

2020-07-26

Publish Date

2020-07-01

Page Start

1

Page End

13

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 66_Issue 166_Pages 1-13.pdf

PDF . 1.0MB

Link

https://avmj.journals.ekb.eg/article_204806.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=204806

Order

11

Type

Review article

Type Code

1,843

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

MICROSCOPIC AND MOLECULAR STUDY OF BABESIA SPP. BABESIA BOVIS AND BABESIA BIGEMINA IN MOSUL CITY-IRAQ

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Article

Created At

23 Jan 2023