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167294

EFFECT OF OOCYTE QUALITY AND CUMULUS CELLS ON MEIOTIC COMPETENCE OF VITRIFIED-THAWED BUFFALO OOCYTES

Article

Last updated: 23 Jan 2023

Subjects

-

Tags

Gynaecology and Veterinary obstetrics

Abstract

The work aimed to study the effect of oocytes quality and the presence or removal of cumulus cells on the meiotic competence of vitrified-thawed immature buffalo oocytes. In experiment 1, Immature buffalo oocytes were classified according to their quality ( group 1: excellent and good, group2: fair & denuded) the groups were vitrified using 20% Ethylen Glycol (EG) and 20% Dimethyle sulphoxide (DEMSO), then thawing after one week and in vitro matured in ISCOVE medium and kept at 38.5°C under 5% CO2 for 22 h. The two groups were compared with the control without vitrification (in-vitro matured oocytes). Experiment 2, excellent and good immature buffalo oocytes were divided into two groups, G1: oocytes with cumulus, G2: oocytes without cumulus then vitrified / thawed as experiment 1, for in vitro maturation assessment. In experiment 1, The excellent and good quality immature oocytes showed significant (p < 0.05) higher in-vitro maturation rate after thawing than fair and denuded buffalo oocytes also vitrification led to loss of oocytes and abnormalities of buffalo oocytes compared to the control. In experiment 2, the presence of cumulus cells was significantly higher in (P<0.05) survivability and maturation rate than the oocytes without cumulus cells. In conclusion, selection of excellent and good quality oocytes with cumulus cells improve post thawing development of vitrified immature buffalo oocytes.

DOI

10.21608/avmj.2020.167294

Keywords

Key words: Vitrification, Buffalo oocytes, Oocytes quality, cumulus cells

Authors

First Name

S.M.A

Last Name

EL-RHEEM

MiddleName

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Affiliation

1 Theriogenology Department, Faculty of Veterinary Medicine, Alexandria University, Egypt.

Email

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City

-

Orcid

-

First Name

omima

Last Name

Kandil

MiddleName

-

Affiliation

Department of Animal Reproduction and Artificial Insemination, National Research Centre, Tahrir Street, Dokki, 12622 Giza, Egypt.

Email

omaima_mk@yahoo.com

City

-

Orcid

-

First Name

IMAN

Last Name

EL-BAWAB

MiddleName

-

Affiliation

1 Theriogenology Department, Faculty of Veterinary Medicine, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

K.K.

Last Name

METWELLY,

MiddleName

-

Affiliation

1 Theriogenology Department, Faculty of Veterinary Medicine, Alexandria University, Egypt.

Email

-

City

-

Orcid

-

First Name

ASS

Last Name

ABDOON,

MiddleName

-

Affiliation

2 Department of Animal Reproduction and Artificial Insemination, National Research Centre, Tahrir Street, Dokki, 12622 Giza, Egypt.

Email

-

City

-

Orcid

-

Volume

66

Article Issue

165

Related Issue

24250

Issue Date

2020-04-01

Receive Date

2020-03-30

Publish Date

2020-04-30

Page Start

87

Page End

100

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_167294.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=167294

Order

7

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023