Corn silk has been used successfully in the traditional management of diseases that are associated with oxidative stress mainly diabetes and diabetic complications such as diabetic nephropathy. The current study aimed to investigate the protective effect of corn silk (CS) powder against diabetic nephropathy. Thirty male Wistar albino rats were randomly divided into five groups (n = 6) as follow; -ve control group fed basal diet, +ve control group fed basal diet and injected with a single dose of Alloxan. Groups 3-5 as the same of group2 and fed on basal diet containing 2.5, 5 and 10% CS powder. Results revealed that CS caused a significant decrease in blood glucose level,urea nitrogen, uric acid, and creatinine. In addition, CS caused a significant increase in insulin level, especially in group 5 which treated with10% compared to other treated group +ve control group. Furthermore, CS caused marked renal and pancreas improvement for damaged that happened due to Alloxan as revealed by alterations in histopathological architectures of kidney and pancreas tissues. In conclusion, CS powder protect rats against Alloxan diabetic nephropathy through its antioxidant, anti-apoptotic, and anti-inflammatory protective mechanisms. So, it may be beneficial to the human beings.