This study was conducted to investigate the effect of cupcakes with different levels of Melissa officinalis L. (Melissa Powder MP) on impaired liver in rats injected with Carbon Tetrachloride (CCl₄) in paraffin oil (1:1) (2 ml/ kg b.w twice a week for 2 weeks. Thirty male mature albino rats, weighing 180 gm were divided into 2 main groups, the first group (6 rats) still fed on basal diet and the second main group (24 rats) had given CCl₄ twice a week for 15 days to induce liver impaired in rats, then classified into 4 groups (6 rats in each group) one of them left as positive control, and the other three groups fed on melissa powder (MP) at levels of 2.5, 5 and 10% for 28 days. Serum liver functions (AST, ALT and ALP), lipid profile (TG, TC, LDL-c, HDL-c and VLDL-c), kidney functions (uric acid, urea and creatinine) and histopathological changes of liver and spleen have been evaluated. The obtained results of hepatic rats revealed that 10% from MP cupcake showed a significant increase in HDL, but with significant decreases in the rest of the analyses referred to, previously, as compared with positive control group.
In conclusion, it was recommended that using Melissa powder (MP) cupcake in daily diets to improve the liver functions.