The main target of this investigation is to study the effect of the prepared pasta supplemented with barley fraction D (FD) (high β-glucan) and quinoa added to pasta products on lipid profile, liver and kidney functions in rats fed on hypercholesterolemic diet. Semolina and maize pasta supplemented with barley F.D (30%, 20%, respectively) and quinoa flour (30%) as well as quinoa pasta supplemented with 20 %F.D. Also, semolina, maize and quinoa pasta were used as control (100%). Sixty male albino rats were put on normal diet for two weeks for adaptation then divided into ten groups (six rats of each). The negative group (control 1) was fed on normal diet, positive group (control 2) was fed on normal diet supplemented with 10% saturated fatty acid (sheep fat), 1% cholesterol and 0.25% cholic acid. From third group to ten groups were fed as the second group + different percentage of semolina, maize, quinoa and F.D. Blood samples of all experimental rats were taken at the beginning, after induction ( 4 weeks) and at final of the experience (10 weeks).Blood samples were collected to determine lipids profile as well as liver and kidney functions. The results showed that positive group (C2) was the major risk factor for induce hypercholesterolemia. The diet fortified by barley F.D that high β-glucan and quinoa flour at different percents improved the lipid profile, liver and kidney functions, and body weight gain, compared with positive group (C2). Histopathological changes were improved. Finally, the last group (G8) which was fed on 80% quinoa + 20 % F.D is similar to a (negative control) in most parameters and outperformed it in High density lipoprotein (HDL).