This study was conducted to evaluate the nutritional and biological activity of some dietary estrogen such as sunflower seeds, barley grains and its effect on adult female albino rats. Chemical proximate, minerals content, quantitation of water-soluble vitamins, tocopherols content and HPLC-MS detection of phytoestrogens of some dietary estrogen were measured. Forty adult female albino rats of Sprague Dawley strain (150 ±5 g) were used and randomly divided into 8 equal groups, one was kept as a negative control group, while the other 5 groups fed on basal diet with dietary estrogen (sunflower seeds, barley grains)( 30 ، 40 % ),  group seven fed a dose of drug(17 βeta-estradiol (E2) in a dose of 2.7µg/day), group eight fed mix from 30 % both of sunflower powder ,barley powder  and drug. The hematological analysis, lipid profile, oxidative stress and hormonal assay were examined.   The obtained results concluded that seeds of sunflower contained the highest crude protein (23.79%) content. The highest crude fiber contents (3.65%) in whole barley, minerals content in whole barley was found relatively lower than the seeds of sunflower. Tocopherol (natural antioxidants) of sunflower seeds achieved the scores of (40.49 mg/100g).Using dietary estrogen improved lipid profile, antioxidant enzymes and liver functions. According to the results, sunflower seeds, barley grains could be used for impaired lipid profile and protection from osteoporosis.