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138135

Enhanced antimicrobial activity of the combination of silver nanoparticles and different β Lactam antibiotics against methicillin resistant <i>Staphylococcus aureus</i> isolates

Article

Last updated: 27 Dec 2024

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Subjects

-

Tags

Infectious disease
Microbiology and Immunology

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) has emerged as a major problem worldwide. Strong synergistic action of antibiotics combined with silver nanoparticles (AgNPs) presents a potential solution for this problem. AgNPs was prepared by Gamma- irradiation method. Eighty staphylococcus spp. were obtained out of 45 different clinical specimens; 21(46.6 %) isolates were identified as MRSA and chosen to test their susceptibility to plain AgNPs, β-lactam antibiotics and mixed solution of AgNPs /antibiotics by disc diffusion method and broth microdilution assay for detection of minimum inhibitory concentration (MIC) as well as minimum bactericidal concentrations. All MRSA isolates were full resistant to amoxicillin, ceftazidime, cefotaxime, ceftriaxone, cefepime, while 3(14.2%) were susceptible to meropenem and imipenem with MIC ≤ 20µg/ml. Plain AgNPs showed potential anti-MRSA activity, the range of inhibition zones of AgNPs ranged from 7.5 to 12 mm. Broth microdilution assay revealed that 8(38.1%) and 5(23.8%) were inhibited at concentration of 31.25 and 7.81µg/ml of AgNPs, respectively. Disc diffusion assay showed the enhancement of activity of amoxicillin/AgNPs, imipenem/AgNPs and meropenem/AgNPs combinations, the fold increase areas ranging between 33.3 to 100, 0 to 166.6 and 0 to 166.6 respectively. Additionally, MIC of amoxicillin in amoxicillin/AgNPs mixture was decreased from more than 5120 μg/ml to reach 1.95 μg/ml in 28.5% of MRSA and 0.97 μg/mL in 19 % of MRSA isolates. The enhanced activity of antibiotics, especially amoxicillin, combined with AgNPs was very high against the resistant isolates, so utilizing combination therapy is one the proposed approaches to treat MRSA bacterial infections.

DOI

10.21608/aijpms.2021.53610.1017

Keywords

<i>Staphylococcus aureus</i>, silver nanoparticles, Gamma Irradiation, β-lactams, Broth microdilution

Authors

First Name

Fatema

Last Name

Abdel-Wahab

MiddleName

-

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Email

fatemabahaaeldin@gmail.com

City

-

Orcid

-

First Name

Nagwan

Last Name

El Menofy

MiddleName

-

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Email

nagwan.elmenofy@azhar.edu.eg

City

-

Orcid

0000-0001-6928-9170

First Name

Ahmed

Last Name

El- Batal

MiddleName

-

Affiliation

Drug Microbiology Lab., Drug Radiation Research Dep., National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt

Email

ahmed.elbatal@eaea.org.eg

City

-

Orcid

0000-0001-5227-9531

First Name

Farag

Last Name

Mosallam

MiddleName

-

Affiliation

Drug Microbiology Lab., Drug Radiation Research Dep., National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt

Email

farag.abomosalam@eaea.org.eg

City

-

Orcid

0000-0003-1784-101X

First Name

Abeer

Last Name

Abdulall

MiddleName

-

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Email

khairy.abeer@yahoo.com

City

-

Orcid

-

Volume

1

Article Issue

1

Related Issue

20376

Issue Date

2021-01-01

Receive Date

2020-12-14

Publish Date

2021-01-01

Page Start

22

Page End

31

Print ISSN

2735-4598

Online ISSN

2735-4601

Article File

AIJPMS_Volume 1_Issue 1_Pages 22-31.pdf

PDF . 602.0kB

Link

https://aijpms.journals.ekb.eg/article_138135.html

Detail API

https://aijpms.journals.ekb.eg/service?article_code=138135

Order

3

Type

Original research articles

Type Code

1,562

Publication Type

Journal

Publication Title

Azhar International Journal of Pharmaceutical and Medical Sciences

Publication Link

https://aijpms.journals.ekb.eg/

MainTitle

Enhanced antimicrobial activity of the combination of silver nanoparticles and different β Lactam antibiotics against methicillin resistant <i>Staphylococcus aureus</i> isolates

Details

Type

Article

Created At

23 Jan 2023