The material of study comprised 21 female patients complaining of hirsut-ism. Their ages ranged from 19 to 35 years {mean 22.6+2.52) and the weight ranged from 53 to 95 kgm (mean 68.2+10.47). Ten carefully se­lected normal matched females were chosen to act as a control group. Both patients and controls were subjected to detailed history, thorough clinical examination, and radiological exami­nations including pelvi-abdominal ul-trasonography and plain X-ray skull. Laboratory investigations were car­ried out in the early follicular phase of the menstrual cycle. Blood sample was taken in the morning and serum separated. A sample of saliva was col­lected at the same time after thorough rinsing of the mouth, and centrifuged at 1500 Xg for 15 minutes. The clear supernatent and the serum sample were preserved at -20°C until time of analysis. Spironolactone was adminis­tered by all patients in a dose of 100 mg/day from 4th to 22nd day of the menstrual cycle. Serum and salivary samples were again obtained. Meas­urement of serum free and salivary testosterone was done by RIA using the COAT-A count testosterone kit supplied by Diagnostic Products Cor-poratin; 5700 West 96th street, Los ANgelos. Hirsutism was graded according to the score of Abraham et al. (1976), while clinical improvement was as­sessed according to Pittaway et al. (1985).