A total of 150 random samples of raw milk were collected from different localities in Assiut city. These samples were examined for the prevalence of P. shigelloides using 2 selective media; Plesiomonas agar (PL agar) and modified Rimler-Shotts medium. The results revealed that 4 (2.7 %) of the examined raw milk were contaminated with P. shigelloides using PL. agar. The incidence of P. shigilloides on modified Rimler-Shotts medium was 2 (1.3%) in the same samples. These findings could be attributed to the neglected sanitary control during the handling and distribution of raw milk. The public health significance of the organism and the recommended sanitary measures were also discussed.