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185430

The Effect of Boiling on Stability of Oxytetracycline and Sulfamethazine Residues in Raw Milk using HPLC Method

Article

Last updated: 04 Jan 2025

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Subjects

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Tags

Biochemistry and Physiology

Abstract

Two hundred raw milk samples (250 ml of each) were collected from small dairy farms, street peddlers and dairy shops in Mansoura Governorate. These samples were screened using Bacillus Subtilis Diffusion Assay for qualitative detection of antibiotics residues; where the percentage of suspected positive samples was 12.5%. High Performance Liquid chromatography – Ultra Violet detector (HPLC- UV) method was developed and validated to determine the amount of oxytetracycline (OTC) and sulfamethazine (SMZ) residues in raw milk before and after boiling. The results revealed that 8.5 % of the raw milk samples were containing (OTC) residues (6.5 % of them exceed MRL) while, (SMZ) was detected in4 % of the raw milk samples (3 % of them exceed MRL). Upon applying heat treatment, the reduction in the (OTC) content in milk boiled for 2 minutes was 30.5% but boiling for 5 minutes was accompanied with 54.1% reduction. On the other hand, the percentage of (SMZ) reduction was 1.7% and 9.5% in milk boiled for 2 and 5minutes respectivelywhich could be attributed to the low heat stability of (OTC) and high stability of (SMZ).

DOI

10.21608/mvmj.2019.22.105

Keywords

(OTC), SMZ, HPLC- UV, Heat treatment

Authors

First Name

H

Last Name

Fathy

MiddleName

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Affiliation

Animal Health Research Institute, Department of Biochemistry, Toxicology and Feed Deficiency

Email

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City

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Orcid

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First Name

Marwa

Last Name

El-Toukhy

MiddleName

-

Affiliation

Mansoura University, Department of Food Control and Hygiene

Email

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City

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Orcid

-

First Name

M

Last Name

Sabery

MiddleName

-

Affiliation

-

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

El-Sherbiny

MiddleName

-

Affiliation

-

Email

-

City

-

Orcid

-

Volume

20

Article Issue

2

Related Issue

22627

Issue Date

2019-06-01

Receive Date

2021-07-25

Publish Date

2019-06-01

Page Start

21

Page End

26

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 20_Issue 2_Pages 21-26.pdf

PDF . 660.7kB

Link

https://mvmj.journals.ekb.eg/article_185430.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=185430

Order

7

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

The Effect of Boiling on Stability of Oxytetracycline and Sulfamethazine Residues in Raw Milk using HPLC Method

Details

Type

Article

Created At

22 Jan 2023