Objective: This study ended to detect the prevalence and public health important of Escherichia coli in chicken carcasses in Mansoura City. Procedures: A total of 100 whole frozen chicken carcasses purchased from supermarkets distributed at Mansoura city were examined to detect Escherichia coli. Results: Out of the 100 examined carcasses, 98 chicken carcasses were contaminated with Escherichia coli with an incidence rate 98%. Conclusion and clinical relevance: This study revealed that chicken meat are highly contaminated with E. coli and play an important role in transmission of E. coli to human, therefore special precautions and hygienic measures must be applied in abattoir during evisceration and dressing of carcasses, and in-home during handling and cooking of chickens to protect consumers from E. coli foodborne infection.