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130401

SUPERIORITY OF PARTIAL 16S rRNA GENE SEQUENCING FOR IDENTIFICATION OF BACTERIAL STRAINS ISOLATED from MEAT PRODUCTS

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Last updated: 22 Jan 2023

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Abstract

Unlike phenotypic methods of bacterial identification, 16S rRNA gene sequencing is an innovative DNA-based technique for the rapid identification of foodborne pathogens. In this study, the ability of both partial 16S rRNA gene sequencing and the API Rapid20E, for identification of 48 different bacterial strains isolated from meat products, plus 2 control bacterial strains, was compared. Genotypically, the partial (770 bp) 16S rRNA gene sequencing was able to identify 38 (76%) isolates to the species level and 11 (22%) isolates to the genus level, whereas only one (2%) isolate could not be correctly identified. Phenotypically, the API Rapid20E could identify 24 (48%) isolates to the specie level, and 14 (28%) isolates to the genus level, while 12 (24%) isolates could not be discriminated at any taxonomic level. The results revealed that partial 16S rRNA gene sequencing was useful in ascertaining the relevance of tested strains; hence, it can be used in food microbiology laboratory for correct and rapid identification of foodborne pathogens.

DOI

10.21608/mvmj.2016.130401

Authors

First Name

Ahmed Mahmoud Mohammed

Last Name

Mahmoud

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Volume

17

Article Issue

2

Related Issue

19229

Issue Date

2016-06-01

Receive Date

2016-04-15

Publish Date

2016-07-01

Page Start

297

Page End

311

Print ISSN

1110-7219

Online ISSN

2682-2512

Link

https://mvmj.journals.ekb.eg/article_130401.html

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https://mvmj.journals.ekb.eg/service?article_code=130401

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60

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Journal

Publication Title

Mansoura Veterinary Medical Journal

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https://mvmj.journals.ekb.eg/

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Article

Created At

22 Jan 2023