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177359

LIGHT SCATTERING SENSOR FOR RAPID DETECTION OF BACILLUS CEREUS IN CHICKEN MEATS

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Last updated: 04 Jan 2025

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Tags

Food Hygiene

Abstract

Bacillus cereus is an opportunistic pathogen causing food poisoning, manifested by diarrhea or emetic illnesses. BARDOT (BActerial Rapid Detection using Optical scattering Technology) directly capturing phenotypic CHARacteristics of colonies following standard agar plating, providing non-destructive, high-throughput analysis and real-time detection of colonies on agar plate without any labeling reagents or probes. This study implement light scattering sensor in detection and identification of B. cereus on genus level in mixed cultures and in artificially inoculated chicken samples using PRM (phenol red mannitol) agar. Software analysis and PCR confirmation showed that BARDOT successfully detect 100% of B. cereus in mixed culture and >90% of artificially inoculated chicken samples. This results demonstrates that BARDOT could be used as a screening tool to identify Bacillus cereus from other pathogens and background flora on PRM agar.

DOI

10.21608//mvmj.2018.19.1212

Keywords

Bacillus cereus, chicken

Authors

First Name

Maha

Last Name

Abdalhaseib

MiddleName

-

Affiliation

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Email

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City

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Orcid

-

Volume

19

Article Issue

1

Related Issue

17732

Issue Date

2018-06-01

Receive Date

2018-03-10

Publish Date

2018-06-30

Page Start

57

Page End

68

Print ISSN

1110-7219

Online ISSN

2682-2512

Article File

MVMJ_Volume 19_Issue 1_Pages 57-68.pdf

PDF . 1.6MB

Link

https://mvmj.journals.ekb.eg/article_177359.html

Detail API

https://mvmj.journals.ekb.eg/service?article_code=177359

Order

5

Type

Original Articles

Type Code

1,268

Publication Type

Journal

Publication Title

Mansoura Veterinary Medical Journal

Publication Link

https://mvmj.journals.ekb.eg/

MainTitle

LIGHT SCATTERING SENSOR FOR RAPID DETECTION OF BACILLUS CEREUS IN CHICKEN MEATS

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Type

Article

Created At

22 Jan 2023