Beta
ManageSearchChatDiscover
228866

Implementation of 16S rRNA Gene and RAPD-PCR for Detection of Ralstonia solanacearum Isolates

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Six isolates of Ralstonia solanacearum were isolated from diseased potato tubers and three isolates of Pseudomonas fluorescens were recovered from the soil rhizosphere healthy potato plants. R. solanacearum isolates exhibited various degrees of wilting on aerial stems of three cultivars of potato. The antagonistic bacterium P. fluorescens had an inhibitory effect against R. solanacearum isolates. Identification of six isolates of R. solanacearum and two isolates of P. fluorescens were carried out using the total DNA isolated in polymerase chain reaction (PCR) analysis, where a region of the 16S rRNA gene had a molecular weight of 1550 bp was amplified by using universal primers. Eight primers (BAR, BAQ, 18, W, BIC 1, I, A9B4 and A9Al0) were used in the differentiation between R. solanacearum as well as the P. fluorescens isolates. Primers produced considerable polymorphism among the studied isolates. The phylogenetic tree based on the similarity matrix was carried using the UPGMA method and revealed the existence of two main clusters, indicating the relationship among the eight examined isolates.

DOI

10.21608/ejp.2011.228866

Keywords

potato, Solanum tuberosum, brown rot, Ralstonia solanacearum, Pseudomonas fluorescens, RAPD-PCR, 16s rRNA gene

Authors

First Name

Said

Last Name

El-Kazzaz

MiddleName

A.

Affiliation

Plant Pathology Department, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria, Egypt

Email

-

City

Alexandria

Orcid

-

First Name

Mohamed

Last Name

El-Sheikh

MiddleName

A.

Affiliation

Plant Pathology Department, Faculty of Agriculture, Damanhour University, Damanhour, Egypt

Email

-

City

Damanhour

Orcid

-

First Name

Elsayed

Last Name

Hafez

MiddleName

E.

Affiliation

Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City 21934, Egypt

Email

elsayed_hafez@yahoo.com

City

New Borg El-Arab City

Orcid

0000-0003-4044-5103

First Name

Samia

Last Name

Madkour

MiddleName

A.

Affiliation

Plant Pathology Department, Faculty of Agriculture, Damanhour University, Damanhour, Egypt

Email

-

City

Damanhour

Orcid

-

First Name

Soha

Last Name

El-Gayyar

MiddleName

M.

Affiliation

Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City 21934, Egypt

Email

-

City

New Borg El-Arab City

Orcid

-

Volume

39

Article Issue

2

Related Issue

32747

Issue Date

2011-12-01

Receive Date

2011-09-29

Publish Date

2011-12-30

Page Start

91

Page End

106

Print ISSN

1110-0230

Online ISSN

2090-2522

Article File

EJP_Volume 39_Issue 2_Pages 91-106.pdf

PDF . 5.5MB

Link

https://ejp.journals.ekb.eg/article_228866.html

Detail API

https://ejp.journals.ekb.eg/service?article_code=228866

Order

7

Type

Original Article

Type Code

1,256

Publication Type

Journal

Publication Title

Egyptian Journal of Phytopathology

Publication Link

https://ejp.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023