Eight isolates of Fusarium ·spp. were isolated from naturally infected khella roots collected from different localities in Minia governorate. The isolates were purified and identified as Fusarium solani. All the isolates were pathogenic to khella plants cv. Balady however, pathogenic isolates varied in their virulence. In general, isolates FK7 and FK8 obtained from Minia were highly pathogenic causing pre- and post- emergence damping-off followed by isolate FK3 which was isolated from Matay, while the lowest virulence was expressed by isolates FK5 and FK6 that was isolated from Samalout. Some chemicals, salts and plant extracts were evaluated for inducing resistance against damping-off in khella under greenhouse conditions. Results indicated that all the tested materials decreased the percentage of pre- and post- emergence damping-off compared to untreated plants. Salicylic acid (SA) (200 ppm), marjorana and neem extract (4%) and dipotassium phosphate (3%) reduced damping-off in khella plants. Also, all tested chemicals significant increased plant survival.