Chia (Salvia hispanica L.) is one of the most important medicinal plants recently introduced to Egyptian cultivation and has significant multiusing in human curing. Fusarium oxysporum, F. roseum, F. semitectum, F. solani, Macrophomina phaseolina, Pythium ultimum, Rhizoctonia solani and Sclerotinia sclerotiorum were isolated from infected plants, showing root and stem rot symptoms collected from different localities of Giza and Fayoum governorates. The isolated fungi significantly realized different (%) of damping- off and root and stem rot.  Rhizoctonia solani, M. phaseolina and F. oxysporum were the most aggressive fungi in the pathogenicity test. Bacillus spp. and Trichoderma spp. gave different degrees of antagonistic effects against the tested pathogens, i.e., F. oxysporum, M. phaseolina and R. solani in vitro. Ascorbic and citric acids at 500 ppm recorded 100% seed germination in vitro. Under greenhouse conditions, Bio-Zeid, T. harzianum, B. subtilis and ascorbic acid gave the highest decreasing percentages of pre, post- emergence damping- off, root and stem rot and maximized healthy survivals. Under field conditions, Bio-Zeid, T. harzianum and ascorbic acid gave the highest decrease percentages of damping- off and root and stem rot and maximized plant survivals in the two experimental seasons, as well as increased plant growth parameters compared with the control (without treatment). Thus, it could be suggested that any of Bio-Zeid as formulated compound, also, ascorbic acid and T. harzianum can be formulated to be used for controlling chia damping-off and root and stem rot.