Many fungal isolates were isolated from naturally rotted cantaloupe fruits. The obtained isolates were purified and identified as Alternaria sp., Fusarium semitectum, F. solani, F. subglutinans-1, F. subglutinans-2 and Rhizopus stolonifer. Pathogenicity test revealed that F. semitectum was the most pathogenic one followed by F. solani then F. subglutinans-1 and F. subglutinans-2. Under laboratory conditions, the efficacy of some bioagents as well as fungicides against the growth of isolated fungi was tested. Under in vitro conditions the antagonistic Bacillus subtilis-1 showed high reduction in the growth of F. solani followed and F. subglutinans-1, meanwhile B. subtilis-2 showed similar effect against the growth of F. subglutinans-1 and F. semitectum, respectively. Moreover, the fungicides Combinex and Basten at concentration of 800 ppm completely inhibited the linear growth of the tested fungi compared with control treatment. Under field conditions, spraying the fungicide Combinex, salicylic acid and the bioagent B. subtilis, alone or in combinations, on cantaloupe plants resulted in significant reduction in natural fruit rot infection with considerable increase in fruit yield and their total soluble solids compared with untreated control.