Ninety wheat promising genotypes were evaluated for their resistance against leaf rust caused by Puccinia triticina under field conditions at Shibin El-Kom and Sadat City during 2014/15 and 2015/16 growing seasons. Evaluation was carried out through disease assessment including final leaf rust severity (FRS), coefficient of infection (CI) and relative resistance index (RRI). Thirty four candidate lines i.e. 3, 6, 14, 15, 16, 18, 20, 21, 22, 23, 24, 25, 28, 29, 30, 31, 32, 33, 36, 37, 38, 39, 43, 48, 50, 52, 55, 62, 77, 84, 86, 87, 89 and 90 out of ninety tested lines were found to be resistant to leaf rust disease and showed acceptable/desirable relative resistance index (RRI) during the two growing seasons. Therefore, these lines can be recommended in Egyptian breeding programs to produce resistant commercial cultivars to leaf rust.