Soybean damping-off caused by Rhizoctonia solani is considered one of the most important diseases in Egypt. Seven isolates were collected from different locations and tested for their virulence on Crawford cv. R. solani (R5) was the highest virulent isolate, which significantly increased pre-and post-emergence damping-off and reduced the survived plants. The ten Trichoderma spp. significantly increased the growth reduction of R. solani in vitro. T. hamatum and T. harzianum were the most effective bioagents which gave the highest growth reduction. All treatments of the biocides Bio-Arc, Bio-Zeid, Bio-4 and Bio-Nagi, T. hamatum and T. harzianum, the green alga (Ulva sp.) and the fungicide Vitavax-200 decreased the incidence damping-off of the four tested cultivars (Crawford, Giza-35, Giza-111 and Giza-83) under greenhouse conditions. Bio-Nagi was the best treatment, which gave the highest survived plants followed by T. hamatum and Bio-Zeid when compared to the untreated control. Under field condition, all treatments decreased damping-off incidence of the four tested cultivars and increased the survived plants during two consecutive seasons (2014 and 2015). Seed yield was positively correlated with plant height, number of pods/plant, 100-seed weight. The fungicide Vitavax-200 was the most effective treatment for controlling damping- off of the four tested cultivars during the growing seasons of 2014 and 15, followed by Bio-Nagi then T. hamatum.