Fusarium wilt of cumin (Cuminum cyminum L.) caused by Fusarium oxysporum f. sp. cumini is a destructive disease, responsible for pre-mature plant death leading to serious economic losses in all cumin-growing areas in Middle and Upper Egypt. The fungus was isolated from the four surveyed governorates (Beni-Sueif, Minia, Assiut and Fayoum). Pathogenicity tests showed that the isolates obtained from Matai County followed by those from Beni Mazar, Minia governorate were the most aggressive and showed the highest frequencies (%) compared to other isolates. Efficiency of some biocides namely Bio-Cure–F (Trichoderma viride, 2x106 spore/cm3), Bio-Cure-B (Pseudomonas fluorescens, 1x108 spore/cm3) and inducer resistance chemicals (chitosan, kinetin and salicylic acid) either individually or in combinations was evaluated in controlling the disease in the greenhouse. Combined treatments between bioagents and the tested elicitors were more effective in reducing the disease incidence than using each treatment alone. P. fluorescens combined with chitosan was the superior treatment in this respect